Journal: Mediators of Inflammation

Article Title: Electroacupuncture Improves Cardiac Function via Inhibiting Sympathetic Remodeling Mediated by Promoting Macrophage M2 Polarization in Myocardial Infarction Mice

doi: 10.1155/2024/8237681

Figure Lengend Snippet: Primer sequences for RT-qPCR analysis.

Article Snippet: Paraffin blocks of heart tissue were processed and cut into 4- μ m sections for immunohistochemistry for TH (1 : 2,000, CST, USA), and together with GAP43 [ , ] (1 : 200, Immunoway, USA), these two antibodies were used to assess cardiac sympathetic innervation.

Techniques:

Journal: Mediators of Inflammation

Article Title: Electroacupuncture Improves Cardiac Function via Inhibiting Sympathetic Remodeling Mediated by Promoting Macrophage M2 Polarization in Myocardial Infarction Mice

doi: 10.1155/2024/8237681

Figure Lengend Snippet: Effect of EA treatment on post-MI cardiac sympathetic nervous system remodeling in mice post-MI. (a, c) Representative TH immunohistochemical staining and analysis of heart tissues in 28 days after MI ( n = 3 per group), scale bar = 50 μ m. (b, d) Representative GAP43 immunohistochemical staining and analysis of heart tissues at 28 days after MI ( n = 3 per group). Scale bar = 50 μ m. (e) The relative mRNA expression of TH in heart ( n = 4 per group). (f) The relative mRNA expression of GAP43 in the heart ( n = 4 per group). Data are expressed as mean ± SEM. ns, no significance, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001.

Article Snippet: Paraffin blocks of heart tissue were processed and cut into 4- μ m sections for immunohistochemistry for TH (1 : 2,000, CST, USA), and together with GAP43 [ , ] (1 : 200, Immunoway, USA), these two antibodies were used to assess cardiac sympathetic innervation.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Mediators of Inflammation

Article Title: Electroacupuncture Improves Cardiac Function via Inhibiting Sympathetic Remodeling Mediated by Promoting Macrophage M2 Polarization in Myocardial Infarction Mice

doi: 10.1155/2024/8237681

Figure Lengend Snippet: Effects of EA treatment on the MI-induced NGF production and the expression of GAP43 and TH post-MI. (a, c) Representative Western blots and quantification of NGF expression ( n = 3 per group). (b, d) Representative Western blots and quantification of TH expression ( n = 3 per group). (e) TH mRNA levels in cardiac tissue at 7 days ( n = 5 per group). (f) Representative images of immunofluorescence staining for NGF/F480+ cell expression in the heart at 7 days ( n = 3 per group). Positive staining for F4/80 is red and NGF is green. Cell nuclei were counterstained with DAPI (blue). Scale bar = 200 μ m.(g) Immunostaining quantification of staining for NGF/F4/80+ cells at 7 days after ( n = 3 per group). (h) GAP43 mRNA levels in cardiac tissue 7 days after ( n = 5 per group). Data are shown as mean ± SEM. ns, no significance, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001.

Article Snippet: Paraffin blocks of heart tissue were processed and cut into 4- μ m sections for immunohistochemistry for TH (1 : 2,000, CST, USA), and together with GAP43 [ , ] (1 : 200, Immunoway, USA), these two antibodies were used to assess cardiac sympathetic innervation.

Techniques: Expressing, Western Blot, Immunofluorescence, Staining, Immunostaining